Six isolates of Phialophota gtegata from soybean were collected from six geographic areas in Egypt Single spore cultures were studied for their variation in cultural characteristics and virulence to soybean. MI isolates varied in their growth rate and colony characteristics on PDA medium at 28⁰C . Maximum growth of the six isolates was at 28°C . The Isolates varied from highly to moderately virulent and were grouped into two pathotypes based on their ability type(I) or inability type(II) to cause chlorosis necrosis and wilt of foliage. Both pathotypes caused internal stem discoloration. All isolates of P. gregab produced toxic metabolites in culture! filtrates, which caused reduction of seed germination and length of radicles. Immersing cut ends of seedlings in autoclaved and unautodaved undiluted crude culture filtrates of diffelent isolates of Phialophora gregata ,caused the small leaves to become flaccid, browning in petiole and veins, then interveinal necrosis was noted , followed by wilting , complete drying and defoliation of the leaves in type (I). Seedlings in culture! filtrate of Type(II) showed, no symptoms.