Anatomical and biochemical parameters have been studied for six mango leave cultivars (Balade, Skare, Zebda, Fagri Klan, Alphonso and Ewais) infested with two mealy bugs, Planococcus citri (Risso) and Icerya seychellarum (Westood). Ammonium humate 3% was used as a bio insecticide to control these pests during the period from April 2020 till March 2021 under field condition. Statistical analysis showed that Ammonium Humate had positive effects on reducing the populations of both I. seychellarum and P. Citri. No infestation by P. Citri was occurred on Alphonso variety while less infested by I. Seychellarum was recorded. Leaf anatomy for this mango cultivar indicated the highest number of resin canals as 14 canals. Skare and Ewais Cvs. had no infestation with I. seychellarum but P. citri was present because a high number of resin canals as 11 and 9-10 canals, respectively. Fagri Klan Cv. had the highest average mean number for I. seychellarum and p.citri populations among treated and untreated mango varieties. Strong positive correlation of Fats, Proteins, Nitrogen, Carbohydrates and Moisture % had found with the I. seychellarum especially on Balade, Zebda & Alphonso Cvs. Likewise, a strong positive correlation occurred with p. citri on Balade and Zebda Cvs. Therefore, Ammonium humate 3% could be used as a bio pesticide in the integrated pest management programs, also, the health of mango crops could be improved according to the biochemical and anatomical parameters of mango plants in this study.