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253276

Physiological, biochemical, and enzymatic implications of “salt and lead” tolerance in Cicer arietinum under hydroponic culture condition

Article

Last updated: 04 Jan 2025

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Abstract

Cicer arietinum is a legume harvested worldwide for its high protein content (20%), which makes it a great meat substitute. Chickpea generally accumulates lead in contaminated soil, particularly when contaminated with Pb(NO3)2. In hydroponic culture conditions, germinated seeds were grown in Hoagland's nutrient medium for 7 d at 23 ± 2°C and 2 h of light. A salt (NaCl) and a metal (Pb) treatment were then applied to the grown plants for 7-8 days while they were in Hoagland's nutrient medium. One test sample was grown in normal Hoagland's mixture of nutrients as control (C), another three were treated with 1 M (T1), 3 M (T2), and 5 M (T3) (Pb) metal concentrations and the remaining three were treated with 20 M, 40 M, and 60 M (salt concentrations), and the remaining three plants were treated with a combination of metal and salt concentrations of 1 + 40 M (T7), 3 + 40 M (T8), and 5 + 40 M (T9) respectively. The control plants showed the best output as compared to all the treatments. The biochemical aspects such as total soluble sugar, and total amino acids provided drastic conditions as treated with salt and metals. The proline, phenol productions were indicating the presence of stress. The mineral contents and the enzymatic activities also contributed positively due to the toxicity contributions of metal and soil toxicity. Thus, it appears that the combination of metal and salt stress has a greater reduction in biomass on the growth of C. arietinum.

DOI

10.21608/ejar.2022.131925.1226

Keywords

Cicer arietinum, Hydroponics, Lead, metal, toxicity

Authors

First Name

Brinda

Last Name

Vaishnani

MiddleName

-

Affiliation

Dept. of Biotechnology, Shri Alpesh N Post Graduate Institute of Science and Research, Anand

Email

brindavaishnani00@gmail.com

City

ANAND

Orcid

0000-0003-3221-5148

First Name

Simran

Last Name

Nathwani

MiddleName

A

Affiliation

Dept. of Biotechnology, Shri Alpesh N Post Graduate Institute of Science and Research, Anand

Email

s.nathwani.1998@gmail.com

City

ANAND

Orcid

0000-0001-6159-2781

First Name

Trusha

Last Name

Baraiya

MiddleName

-

Affiliation

Dept. of Biotechnology, Shri Alpesh N Post Graduate Institute of Science and Research, Anand

Email

trushabaraiya11@gmail.com

City

ANAND

Orcid

0000-0001-8307-7916

First Name

Jitendriya

Last Name

Panigrahi

MiddleName

-

Affiliation

Department of Biotechnology, Shri Alpesh N Patel PG Institute of Science and Research, Anand affiliated to Sardar Patel University

Email

jitenp2001@gmail.com

City

ANAND

Orcid

0000-0002-9658-1596

Volume

100

Article Issue

4

Related Issue

36059

Issue Date

2022-12-01

Receive Date

2022-04-07

Publish Date

2022-12-01

Page Start

483

Page End

498

Print ISSN

1110-6336

Online ISSN

2812-4936

Link

https://ejar.journals.ekb.eg/article_253276.html

Detail API

https://ejar.journals.ekb.eg/service?article_code=253276

Order

6

Type

Original Article

Type Code

1,041

Publication Type

Journal

Publication Title

Egyptian Journal of Agricultural Research

Publication Link

https://ejar.journals.ekb.eg/

MainTitle

Physiological, biochemical, and enzymatic implications of “salt and lead” tolerance in Cicer arietinum under hydroponic culture condition

Details

Type

Article

Created At

22 Jan 2023