The performance of five cultivars of Egyptian cotton, Giza 80, Gin 83, Giza 85, Giza 89 and Giza 90 were evaluated during the two successive seasons, 2001 and 2002 at four locations in Middle and Upper Egypt (Salad, El-Minia, 8eni-Soulef and 9-Falyum). The effect of genotypes, years, locations, genotypes by locations and genotype by location by year interections were highly significant for all studied traits (dl %, protein %, seed index and seed cotton yield). While the effect of the interaction between genotypes and years was insignificant only for seed index trait. Also the effect of years was insignificant for protein %. Giza 85 significantly surpassed all the studied cultivars in oil %, while Giza 89 gave the less values in oil 16, protein % seed India and seed cotton yield (k/f) characters. Giza 90 surpassed all studied cultivars In protein % and seed cotton yield (k/f) at all studied locations. The seed cotton yield (k/f) and oil % traits were significantly differed the highest obtained (ran genotypes grown at Beni-Soule( region. This may be due to the environment of geni-Soulef region that helped to improve these characterisdcs. Positive and significant correlations were found among (seed oil % and seed index), between (seed cotton yield and seed inched and between (di % and seed cotton yield). Insignificant negative correlation was recorded between seed Index and protein %. The results indicated that the correlation was positive and significant for Giza 85 between of % and protein %. It recommendation by this correlation in the breeding program to improve seed quality and seed cotton yield.