The present study aimed to examine the bacteriological bad in water of input-irrigation canal and fishponds of Abbassa fish farm as well as in gills, musdes and intestine of reared Nile tilapia, (Oreodrromis Motleys). Also, the study comprised determination of physico-chemical characters and some heavy metals (Cu, Fe, Mn & Zn ) of input-inigation canal and fishponds water at the same periods to evaluate their hygienic conditions for good fish production. The samples of water and fish were examined one time every month. The total bacterial counts, fecal coliform, E. c^-oli, Salmonella sop., Staphykroccus aureus and Aeromonas sop. in the fishponds water were higher than those in the canal water. The maximum counts of total bacteria and Staphfioaccus auteus were recorded in July. Coliform and Salmonella sop. recorded maximum counts in August, while, E co✓i recorded maximum counts in June, and Aeromonas spp. showed the maximum counts in April. Moreover, the examined fish organs revealed that the total bacterial counts, colWorm, E coli, Salmonella spp., Staphylocoaus emetics and Aeromonas sop. in the intestine > gigs > muscle throughout the studied rearing season (started from April to November). The physico-chemical characters (water visibility, dissolved oxygen, pH, total ammonia, unionized ammonia, nitrite-nitrogen, nitrate-nitrogen, total phosphorus and total hardness) and concentrations of the studied heavy metals (Cu, Fe, Mn & Zn) in water of fishponds were more different from those of input canal at the most time of the experimental period. However, the conditions of input-Irrigation source canal water and fishponds water of Abbassa fish farm during the period of study were safe for fish rearing and the produced fish were safe for human consumption.