The experiments were conducted in the Baramoon Experimental Station. Dakahlia Governorate, Egypt, during two successive seasons of 2001/2002 and 2002/2003. The aim of this study is to investigate the response of Borage plants to nitrogen fixing bacteria plus phosphate dissolving bacterial fertilizers and their combinations. Moreover, investigating the effect of organic manure on growth and diemical composition of Borage seed oil The levels of organic manure were 0, 12, 18 and 24 m3/fed from compost. Strain of nitrogen fixing bacteriaa "Azospirillum sp." Under the commercial name of "Nitrobein" and phosphate dissolving bacteria "Bacillus mogateriunt under the commercial name of "phosphorein" were applied to Borage seedlings after one month of sowing. The soil inoculation was repeated twice every month. Results showed that biofertilization plus organic manure improved plant growth characters expressed as plant height, number of branches, fresh and dry weight/plant and /plot of both leaves and flowers, seed yield, fixed oil percentage and 8-linolenic acid of different treatments. The best results were obtained by inoculation with Nitrobein plus phosphorein and organic manure at rate 24 m3/fed combination due to the action of different bacteria strain on availability of the elements. Data also revealed that biofertilizer is a biological technique for reducing the dose of mineral fertilizer to Borage plants.