Two field trials were carried out at Sakha Research Station, Kafr El-Sheikh Governorate in 2001/2002 and 2002/2003 seasons to study the effect of six treatments represent three nitrogen sources [Urea (46% N), Ammonium Nitrate (33.5% N) and Amounim Sulfate (20.6% N)} and two nitrogen levels (69 and 115 kg N/fed). Sugar beet T;a7ella variety was planted. Treatments were distributed in a complete randomized block design in four replications. The results showed that nitrogen sources significantly affected sugar yield and alpha amino nitrogen percentages while root length, root diameter, root fresh weight/plant, extractability percentage and root yield were not significantly affected by nitrogen sources. Nitrogen levels caused a significant effect on root diameter, root fresh weight/plant, sucrose %, root yield and alpha amino nitrogen % in both seasons and sugar yield in the Iⁿ season.