This study was conducted to investigate the effect of two sowing dates (first week of September and October) and the three strawberry varieties, i.e., Rosalinda, Camarosa and Selected on infestation with the two-spotted spider mite, T. urticae at South of Tahreer Province during the two seasons 2001/2002 and 2002/2003. The relationship between leaf area of the three varieties and mite infestation was also studied. Results revealed that during the two seasons, plants of the second date harboured the highest number of moving stages for the three tested varieties. In addition, Camarosa followed by Selected varieties were infested with highest numbers of both stages at the two planting dates, while Rosalinda came next in this respect. On the other hand, results revealed that during plant vegetative stage, strawberry harboured the highest numbers from both eggs and moving stages, while moving stages decreased gradually during both flowering and fruiting stages. The infestation became fewer at the end of growing season. Infestation with mite different stages usually increased with the increase of leaflets area. The leaflets of Camarosa variety which represent the widest leaflets harboured the highest number of both eggs and mite moving stages.