Nanotechnology's application in biomedicine has piqued the curiosity of all authorities throughout the world. Different fungus species and aflatoxins were recovered from rabbit feed at significant quantities up to 300 ppb in this investigation. Selenium nanoparticles (Se-NPs) were generated using a chemical process, described briefly, and utilized to reduce the harmful effects of aflatoxicosis in rabbits. Biochemical parameters in tissues and sera of all aflatoxicated rabbits revealed that it caused the development of reactive oxygen species (ROS), an increase in nitric oxide levels, and malonaldehyde levels. While Total Antioxidant Capacity (TAC) activities are reduced, additional antioxidants in rabbit homogenate liver tissues will be found. In aflatoxicosis, decreased serum retinol and a-tocopherol were also found. For aflatoxicated rabbits, oral treatment of SeNPs and thyme restored these alterations to normal levels. Furthermore, SeNPs and thyme triggered the breakdown of AFB1 residues in liver tissues, suggesting that SeNPs and thyme can be introduced to animal feeds as antioxidants with good effects on animal health and mycotoxins removal.