The development of wilt symptoms is the main criterion for the identification of such isolates A oxyspotum f. sp. vasinfectunz This biological test is time consuming and sensitive to temperature changes. Therefore, differentation between A axyspotum 1. sp. vasinfeaum isolates from the recently isolated damping-off producing isolates of A owsporum are being explored by RAPD-PCR using any of the primers d (CGTGCGGGAA)-3 or d (AAGAGCCCGT)-3 or d (AACGCGCAAC)-3 or d (CCCGTCAGCA)-3 by RAPD-PCR. The method was found sensitive, aozurate, practical and time saving in recognizing F arysporum f. sp. vasinfectum isolates, which showed a very high degree of similarity. In =bast, the damping-off causing F. oxysporum isolates tested showed different dusters with a low overall similarity.