The vegetable crops are acutely sensitive to pest attacks, disease proliferation, and weed development. To control these pests, farmers resort excessively to various chemical plant protection products. Therefore, this study aims to measure the intensity of pesticides use and to estimate the phytosanitary pressure according to the phytosanitary practices of market gardeners in the region of Biskra, South-East of Algeria. Questionnaire surveys were conducted among 96 randomly selected market gardeners in Biskra during two farming campaigns 2016/2017 and 2017/2018. Results show that out of 70 different active substances, eighteen are mostly applied on seven inventoried vegetable species. Insecticides and fungicides are the most widespread. The average values of the calculated treatment frequency index are ranged from 0.80 to 30.45. The phytosanitary pressure index is particularly dangerous for tomato, eggplant, and chili pepper, with values ranging from 4.03 to 4.29 and 8.25, respectively. In conclusion, the surveyed areas were found to be experiencing considerable phytosanitary pressure, which would have a negative impact on the environment and human health.