The present study was conducted to study the relationship between level of serum cholesterol, immunity, and physiological and productive performance for some local strains of thicken. A total of 400 Oneday- old female chicks from four local strains of chicken, Dandarawi, Dokkl 4, Batadi and Sinai (100 female chicks of each) were used to estimate some productive, physiological and immunological parameters as indicators to produaive performance. The chicks were reared on deep litter, under natural day light and environmental temperature. The feed and water were provided ao bb. The experiment continued for 6 months. The obtained results were as following: 1.Sinai strain female chicks had higher body weight and feed consumption at almost ages. 2.Serum cholesterol , serum LDL and serum HDL were higher in Dokki strain chicks at all ages, Dandarawl strain gave the lower serum levels of cholesterol, LDL and HDL . 3.Liver cholesterol, LDL and HDL were lower in Dandarawi strain, liver triglyceride, and total lipids were lower in Dandarawi and Baladi compared with other strains at almost ages. 4.Serum triglyceride and total lipids were lower in Baladi chicks at all ages. 5.Sinai strain chicks were higher in serum total protein, albumin, and globulin. 6.With regard to immune response, we found that Baladi strain chicks gave the best response against sheep red blood cells (SRBC) at early age (12, 16 weeks). 7.At late ages (20, 24 weeks), Dokki strain presents higher response against SRBC. The relationship between serum cholesterol level, immune response, and physiological and productive performance for Dandarawi and Baladi strains may explain the importance of selection for decrease level serum and liver cholesterol with increase the immune response before egg production to Improve the production of eggs with less cholesterol level and increasing the immunity of these local strains in the future.