One hundred and eight isolates of P. tribeina , causing wheat leaf rust were collected across eight governorates i.e. Alexandra, Ismailia, Dakhlia, Beheire Dernietta, Beni-Swief, Sharkia and Fayoum during 2005/2006 and analyzed for virulence to 47 Thatcher near-isogenic wheat lines, each carrying a single gene for resistance to leaf rust. Of the 68 virulence phenotypes identified among 108 isolates,39% were collected from Alexandra, 37% from Ismailia, 6% from Dakhlia, 5% from each of Behelre Demietta, and Beni-Swief, 4% from Sharkia and 1% from Fayoum. The most frequent were T- virulence phenotype groups (40% of ffie total 'Sables), P-virulence phenotype groups (18%), F-witilence phenotype groups (11%) and M-virulence phenotype groups (6%). Moreover; the most virulent isolates were 777T, and TTTS (91.5% of the tested genes were susceptible), followed by isolates FS7T, PM77, TRTT AND 7TKT (89.4% Virulence) . The frequency of virulence was greater than 90% to 147, Lr22b, Lr46, less than 50% to Lr2a, Lr36, Lr39, 1,45 and between 50 and 90% to the rest Lr genes. Moreover, the 1,37 was the most effective gene (85.2% efficacy) followed by Lr39 and 1,45 (66.7% efficacy, each) then Lr2a and Lr 36 (53.7%, each). The lowest isolates with virulence to these genes and the high level of resistance that they confer indicate that genes Lr37 and Lr39 would provide effective resistance in a breeding program