Beta
ManageSearchChatDiscover
218399

PREPARATION OF PEROXIDASE CONJUGATED AND NON- CONJUGATED ANTISHEEP IgM IMMUNOGLOBULIN

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Peroxidase conjugated and non-conjugated antisheep immuno-globulin-M (IgM) were prepared from rabbit hyperimmune serum. The optimal dilutions of both products were determined by checkerboard ELISA method which proved their efficacy. Their titres were 1:2000 and 1:100, respectively. Both peroxidase conjugated and non-conjugated antisheep IgM, in comparing with imported standardized product were applied on 28 serum samples collected from sheep infected with Rift Valley Fever virus (RVFV) to detect IgM antibodies against RVFV using both indirect ELISA and IgM capture ELISA. The results revealed that non-conjugated antisheep IgM using IgM capture ELISA was superior to peroxidase conjugated antisheep IgM using indirect ELISA for detection of IgM antibodies in sheep.

DOI

10.21608/ejar.2007.218399

Authors

First Name

TARADI A.

Last Name

SAYED

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt

Email

-

City

-

Orcid

-

First Name

KAERM E. Z.

Last Name

HASSAN

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt

Email

-

City

-

Orcid

-

First Name

SAFAA, A.

Last Name

WARDA

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt

Email

-

City

-

Orcid

-

First Name

MOHAMED M.

Last Name

TAHA

MiddleName

-

Affiliation

Central Laboratory for evaluation of Veterinary Biologics, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt

Email

-

City

-

Orcid

-

First Name

AHMED M.

Last Name

DAOUD

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt

Email

-

City

-

Orcid

-

Volume

85

Article Issue

2

Related Issue

30092

Issue Date

2007-07-01

Receive Date

2006-06-25

Publish Date

2007-07-01

Page Start

763

Page End

771

Print ISSN

1110-6336

Online ISSN

2812-4936

Article File

EJAR_Volume 85_Issue 2_Pages 763-771.pdf

PDF . 3.2MB

Link

https://ejar.journals.ekb.eg/article_218399.html

Detail API

https://ejar.journals.ekb.eg/service?article_code=218399

Order

29

Type

Original Article

Type Code

1,041

Publication Type

Journal

Publication Title

Egyptian Journal of Agricultural Research

Publication Link

https://ejar.journals.ekb.eg/

MainTitle

PREPARATION OF PEROXIDASE CONJUGATED AND NON- CONJUGATED ANTISHEEP IgM IMMUNOGLOBULIN

Details

Type

Article

Created At

22 Jan 2023