Two field trials were carried out at Mattana Agricultural Research Station, Qena Governorate in two successive growing seasons (2004/2005 and 2005/2006) to evaluate the performance of four sugar cane varieties (G.T 54-9, ph.8013, 695-19 and F.161) under three blofertilization treatments (Leman, Azotobacter and Azospirillum) and three seeding rates, i.e. 8400, 12600 and 16800 of 3- budded cane cuttings arranged in 1, 1.5 and 2 rows, respectively. A split-split plot design with three replications was used in both seasons, the three biofertilizers treatments were allocated in main plots, while the three seeding rates were distributed in the sub plots and the four varieties were randomly distributed in the 2.4 order plots. The results indicated that bacterial inoculation of sugarcane significantly improved stalk height, purity percentage and sugar recovery, number of millable cane/fed cane yield in both seasons. However, bacterial inoculation significantly improved stalk diameter and sucrose in the second season only. Inoculation with Azotobacter gave the highest values of cane and sugar yields and sugar recovery %. Planting sugarcane using 16800 cane setts/fed in two rows attained significantly higher stalk height, purity percentage, number of millable cane/fed and cane yield in both seasons. Whereas, sucrose and sugar yield were Insignificantly affected by seed rate in both. seasons. However, seed rate significantly affected stalk diameter and sugar recovery% in the second season only.The results indicated that the tested sugarcane varieties differed significantly in all traits under study. G.T 549 variety recorded the highest sucrose and purity percentages and sugar recovery%. while, P.h 8013 variety surpassed all varieties in cane and sugar yields. The effect of the 1° order and 2°3 interactions on all studied traits were insignificant In both seasons.