The effect of protein levels (15, 25 and 35% protein) on spawning performance of Nile tilapia (Oreochromis nlloticus) were investigated in outdoor tanks. Spawning was monitored; the data on egg characteristics (fecundity and egg size) were recorded. The results indicated that increasing the protein level fed from 15% to 35% increased significantly the body weight and fecundity of 0. niloticus females. On the other hand, supply of inadequate protein (15% protein level) resulted in prolonged intervals between spawning. Means of egg diameter between and within treatment were not significantly different. The present study shows the important of high-protein diet in enhancing reproductive performance of Nile tilapia. Also, these findings give fish farmers an option in the management of feeding of Nile tilapia brood stock. Based on our results, we suggest that a minimum of 35% protein be included in the diet female Nile tilapia brood stock