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213783

PROGENY TESTING OF CONVERTED MATERNAL (XY) GENOTYPE AFTER SEX REVERSE TO PRODUCE SUPER MALE (YY) IN OREOCHROMIS NILOTICUS EXPERIMENTALLY

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Last updated: 24 Dec 2024

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Abstract

The present study is continuation to the first step in a comprehensive program for producing "supermale_s" (YY) of Oreochromis niloticus in Egypt to produce all male tilapia for increasing fish production and prevent unwanted reproduction in ponds. Sexually undifferentiated fry after yolk sac absorption period were feminized using 17-0 ethynylestradiol incorporated into the feed. Fry were reared in hapas suspended In an earthen pond and fed for three time durations, 3, 4 and 5 weeks on three different dosages of the feminizing stimulating agent (FSA) 50, 100 and 150 mg/kg/ feed. Growth of fry was affected by the dosages used. Average weight of fry that were fed on higher doses of 17-13 ethynylestradiol supplemented feed was significantly higher than those fed on lesser amounts for the same period. The effect of 17­p ethynylestradiol as a (FSA) was highly detectable among a niloticus fry (1 generation) that were fed on 150 mg/kg (FSA) -treated feed for 5 weeks. Progeny test was adopted to estimate the mean percentage of phenotypic females MPF that reached 92.0 2.3% when fry were fed for 5 weeks on 150 mg/kg (FSA) treated -feed. MPF decreased with decreasing the quantity of (FSA) in the supplementary feed. The length of feeding period when interacted with the (FSA) dose in the supplementary feed had an appreciable effect on sex reverse among progenies of 0.  nilolias. After rearing of the sex reversed fry to sexual maturity and crossing normal males (xy) to the undifferentiated pseudo- and normal females (t­xy and roc), twenty four converted pseudofemales with female phenotype and xy male genotype could be differentiated by progeny testing of their offspring (2"d generation) that consisted male percentage varying from 69 to 79% males exceeding the normal ratio. Hypothetically, one third of these males should be super males of the "rr genotype and two thirds should be normal males. Further research by crossing these males after sexual maturation to normal females and testing their progeny will prove' or disprove this hypothesis.

DOI

10.21608/ejar.2007.213783

Keywords

sex reversal, Oreochramis nlloticus, Feminization

Authors

First Name

MOHAMED E.

Last Name

FARAG

MiddleName

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Affiliation

Genetics Department Central Laboratory for Aquaculture Research, Abbassa, Sharkia, Egypt

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Orcid

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First Name

GAMAL A.

Last Name

MOHAMED

MiddleName

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Affiliation

Reproductive physiology and hatchery Departement Central Laboratory for Aquaculture Research, Abbassa, Sharkia, Egypt

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Orcid

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First Name

HUSSEIN A.

Last Name

ELGHOBASHY

MiddleName

-

Affiliation

Genetics Department Central Laboratory for Aquaculture Research, Abbassa, Sharkia, Egypt

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City

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Orcid

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First Name

AHMED M.

Last Name

KHATER

MiddleName

-

Affiliation

Reproductive physiology and hatchery Departement Central Laboratory for Aquaculture Research, Abbassa, Sharkia, Egypt

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Orcid

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Volume

85

Article Issue

1

Related Issue

29635

Issue Date

2007-03-01

Receive Date

2006-03-02

Publish Date

2007-03-01

Page Start

317

Page End

327

Print ISSN

1110-6336

Online ISSN

2812-4936

Link

https://ejar.journals.ekb.eg/article_213783.html

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https://ejar.journals.ekb.eg/service?article_code=213783

Order

26

Type

Original Article

Type Code

1,041

Publication Type

Journal

Publication Title

Egyptian Journal of Agricultural Research

Publication Link

https://ejar.journals.ekb.eg/

MainTitle

PROGENY TESTING OF CONVERTED MATERNAL (XY) GENOTYPE AFTER SEX REVERSE TO PRODUCE SUPER MALE (YY) IN OREOCHROMIS NILOTICUS EXPERIMENTALLY

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Article

Created At

22 Jan 2023