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TRIALS FOR PREPARATION OF COMBINED INACTIVATED VACCINE AGAINST RESPIRATORY VIRAL DISEASES OF POULTRY (ND-IB-ILT)

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Last updated: 24 Dec 2024

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Abstract

One hundred and seventy susceptible chicks, 21 days old, were divided into four main groups. The 15`, 2nd and 3`d groups were vaccinated by three different. designed combined oil inactivated vaccines against Newcastle (ND), Infectious Bronchitis (IB) and Infectious Laryngo-tracheitis (ILT) diseases viruses while the 4thgroup was kept as control. Half of each vaccinated group was boostered three weeks later by the corresponding prepared vaccine. Blood and serum samples were collected for evaluation of cellular and humoral immune responses. Estimation of cell mediated immunity was carried out using lymphocyte blastogenesis and phagocytic activity. Better response was obtained in the Pt group at 2 weeks post initial vaccination and 1 week after boostering. There is a decrease in the heterophil/lymphocyte ratio in group 1 in comparison with the highest ratio in control group. The humoral immune response was detected by haemagglutination inhibition test for NDV,,serum neutralization test (SNT) and ELISA test for IBV and SNT for ILT virus. The highest value of the antibody titre was recorded for the rt designed vaccine followed by the 2nd designed vaccine and finally the 3rd designed vaccine.

DOI

10.21608/ejar.2007.213781

Authors

First Name

MOHAMED A. A.

Last Name

ABD EL-KHALECK

MiddleName

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Affiliation

Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt

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Orcid

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Volume

85

Article Issue

1

Related Issue

29635

Issue Date

2007-03-01

Receive Date

2006-05-14

Publish Date

2007-03-01

Page Start

307

Page End

316

Print ISSN

1110-6336

Online ISSN

2812-4936

Article File

EJAR_Volume 85_Issue 1_Pages 307-316.pdf

PDF . 2.3MB

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https://ejar.journals.ekb.eg/article_213781.html

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https://ejar.journals.ekb.eg/service?article_code=213781

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Original Article

Type Code

1,041

Publication Type

Journal

Publication Title

Egyptian Journal of Agricultural Research

Publication Link

https://ejar.journals.ekb.eg/

MainTitle

TRIALS FOR PREPARATION OF COMBINED INACTIVATED VACCINE AGAINST RESPIRATORY VIRAL DISEASES OF POULTRY (ND-IB-ILT)

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Article

Created At

22 Jan 2023