The present study was carried out on the Upper Egypt Long-Staple cottons to assess an appropriate new standard count for comparing the strength level of Upper Egypt cottons tested in the breeding, maintenance and regional evaluation and other research programs. Two Egyptian commercial cotton varieties belonging to Upper Egypt Long-Staple category namely; Giza 80 and Giza 90 were used in this study. All cotton samples were processed into three yarn structures; two nominal counts 40s with 3.6 and 4.0 twist multipliers, and a third nominal 60s with 3.6 TM. The results demonstrated that the proposed standard count 40s with 4.0 twist multiplier being the least in yam count, lea strength variation, and number of end breakages and gave the optimum quality. Furthermore, it reduces the time per test. Consequently, it is expected the order of ranking of different strains which could affect selection is changed and led to less rejected samples. The suggested count correction factor to be used is 1.2 units to be added if the yarn is finer and subtracted if it is coarser than the nominal count, from the lea strength.