Pollen supplements with addition of ' different lipid concentrations (3,5 and 7% lipid) were tested for honeybee colonies feeding preference. The quantities of examined diets consumed by the treated colonies which fed with four pollen substitute diets: Yeast (control), dried medical yeast with 3% food oil (A), yeast with 5% food oil (B) and yeast with 7% food oil (C) were evaluated. The highest mean of food consumptio56.51 %diet obtained from (C) , followed by (A) (52.84%) and (8)(38.65 %) , while the lowest mean of food consumption (25.37%) was obtained from (Y) diet .The study showed significant differences among the mean quantity consumption obtained from all treatment and control diet. The study discuss the mean values of total lipid in body of larvae, emergency nurses, forager worker and larvae and adult drones produced from treated colonies. The experiments showed no significant differences were found among the mean lipid values obtained from all treatment and control of larvae and emergency honeybee workers. The highest total lipid values in body bees were obtained from those fed on (A) of nurse bees and larvae and adult drones, whereas (8) resulted was the highest total lipid values in body of forager bees. The study showed also significant differences among the mean lipid values obtained from all treatment and control diet of nurse and adult honeybee workers, also larvae and adult honeybee drones.