The present study was carried out to investigate the effect of induced Malathion toxicity on Swiss mice and the role of some antioxidant (AD3E and ascorbic acid) for protection. Histopathological examination revealed haemorrhage, severe vascular and necrotic changes prominent in the liver and heart. Analysis evoked significant increase of AST and ALT enzymes in experimental Malathion toxicity group, while, the treated group revealed mild histopathological changes and an excellent improvement in enzyme parameters.