A total of 100 fresh faecal samples and faecal swabs were collected from seagulls and wild birds; 46% were positive for Escherichia coli. Serological identification of isolated strains of E.coli from seagulls and wild birds showed that they belonged to "7" different "O" serogroups which were O1, O18, O55, O126 from seagulls and O26, O55, O114 from wild birds. The antibiogram of the isolated serogroups of E.coli revealed that all tested serogroups of E.coli were sensitive to cefaperazone except serogroup (O1). On the contrary, with ceftazidine and subctam-ampicillin all tested E.coli serogroups were resistant. Meanwhile, the examined strains gave variable results with the remaining used chemotherapeutics. Plasmid analysis of seven E.coli isolates demonstrated significant homology between them which ranged from 30-50 Kbp.