The aims of the present research are to analyze the polymorphism between three goat breeds Baladi, Hybrid (BaladiDamascus), and Damascus breeds by Biochemical and advanced molecular assay. A total of 15 samples from three goat breeds; Baladi (n=5), Hybrid (n=5), and Damascus breeds (n=5) were used. The accession no. of MW654246, MW654247, and MW654248, with Baladi, Hybrid, and Damascus goat breed, respectively. The deposited sequences of objects are part of intron 3, and exon 3 is fully sequenced with three breeds, according to the findings. The frequency of the Myostatin gene was the same in all three breeds. Also, the deletion of C and A in two SNPs of 259 and 260, respectively with all breeds were showed. While the convert case with Baladi breed from A to T in the SNP of 236 is the case in Hybrid breed. Damascus breeds the convert of T to C in the SNP with 248. These results agree with the molecular genetics data output the higher similarity values between the three goat breeds were found. The value of similarity between samples Baladi and Hybrid goat breed was 0.92%. And between Damascus and Hybrid goat breeds were 0.74%. Finally, the value of similarity was lowest between Baladi and Damascus goat breed of 0.62% using analysis of plasma. The three goat breeds investigated exhibited low variation in the Myostatin gene; these results suggest that the Baladi breed has a higher amount of genetic material (DNA) that will be passed down to future generations than the Damascus breed.