This Work aimed to use pigments extracted from banana peel as natural waste source to dye some Egyptian cotton fabrics. Alkaline extracted solution using 0.1% NaOH was analyzed by high performance thin layer chromatography (HPTLC), analysis technique. The alkaline solution was applied to the premordated bleached and mercerized cotton fabrics made from Giza 89 and Giza 83 cotton varieties. Ferric chloride, copper sulphate and potassium dichromate were used as mordents. The factors affecting the fixation of the extracted dye with cellulose was studied. The treated and untreated fabrics samples ware tested for their mechanical properties expressed as tensile strength (g/tex) and elongation %. Dyeing performance in terms of color parameters (K/S, L*, a*, b* and ΔE), and fastness properties (wash, perspiration, and light fastness) were studied. The samples show high tensile strength, high color strength, and high fastness properties. These results are very important for industrial application with the production of a natural dye as an inexpensive source from banana peel as a by product. Another objective is to increase the production of eco-textile garments with a good price for the Egyptian customers .