Two field experiments were carried cut in 2006/2007 and 2007/2008 seasons at Malawi Agricultural Experimental Research Station El Minia Governorate to study the effect of nitrogen soil fertilization level (60. 90 and 120 kg N/fed) and foliar fertilization of sulphur in the form of Calciume Poly Sulfide (30%) at concentration of 6 orn/L. which was applied (once at 70 days age, twice at 70 and 85 days as well as three times at 70, 85 and 90 days after sowing in addition to foliar spray with distilled water as control) on sugar beet Desperiz Poly N variety. The results indicated that: Increasing nitrogen level up to 120 Kg/fed significantly increased growth of sugar beet root measurements (length, diameter and fresh weight) and its content from N, P, K, SO., and all amino acids (except cystein which increased more by 90 kg N/fed) as well as yield of roots. However fertilization sugar beet plants by 90 kg N/fed significantly increased root quaky percentages (total soluble solids, sucrose and purity) in addition it gave the highest sugar yield (ton/fed). Foliar spray with sulphur solution twice or three times significantly increased d'ameter, fresh weight, yield of root and accumulation N, P, K, SO.,. while, some amino acids were increased and others were decreased in sugar beet root. Sulphur fertilization significantly decreased root quality measurements and it have insignificant effect on sugar yield. Fertilization sugar beet by 90 Kg N/F in combination with sulphur foliar spray three times during growing season obtained the greatest Increases in root and sugar yield.