Many factors can be affected or neutralizes inhibits the toxin activity of the bee venom as exposure honey bee colonies to different diseases or parasites affects on venom active proteins and differentiation in the amino add concentrations led to harmful of the venom components as the ailment by Varroa mite or Nosema diseases. Bee venom extracted from infested nurse bee workers with Varroa mites showed decreases in amino acids concentration particularly with Glutamic acid, Methionine and Phenyl Alanine certain amino adds. While Asparagine,Q-Aminobutaric acid, Aniline, Tyrosine, Methionine, Cystine, Isoeudne, Leudne, Phenyl Alanine, Tryptophan and Lysine represented momentum concentration decreased with the nurse bees infected with the Nosema disease which consider one of the most affects diseases to honey bee products.