The present work was carried out in a trial to overcome the possible obstacles which may face the evaluation of egg drop syndrome (EDS) vaccine through the application of the challenge test using the virulent virus. It spots the light on the relationship between the levels of EDS antibodies in vaccinated hens and the quality and quantity of laid eggs from these hens. Live attenuated chicken embryo fibroblast cell culture (CEF) and inactivated duck egg EDS vaccines were used to such purpose where they were used to vaccinate 2 groups of hens at 4 and 16 weeks of age. The induced antibodies in vaccinated hens were followed up using hemagglutination inhibition test (HI) and serum neutralization test (SNT) revealing that all vaccinated hens exhibited high levels of specific EDS antibodies confirming the production of good quality and quantity of eggs.