The purpose of this study was to quantify ginning efficiency and fiber quality for regional cotton cultivars ginned on commercial style equipment. Results should allow plant breeders and ginners to consider improvements in cotton cultivars that are in response to ginning conditions that more nearly reflect commercial ginning practices. Therefore, the seed cotton of Giza 88 and Giza 87 growing in [Kafr El-Sheikh - Kafr El-Dawar - Etay El-Barood - Damietta], Giza 86 and Giza 85 growing in [El-Sharkia - El-Gharbiya - El-Dakahliya - El-Monofiya], Giza 80 and Giza 90 growing in [Sohag - El-Minia - Beni-Sueif] were used in this study. The results obtained could be summarized as follows: 1. Growing Giza 80 and Giza 90 in Beni-Sueif location exhibited the highest gin-stand capacity, which could be explained by the environmental conditions affected fiber properties and reduced the fiber strength, fiber maturity, fiber fineness expressed as maicronaire value and that led to make ginning more easily and increased gin stand capacity. 2. No significant differences in ginning efficiency and fiber properties due to growing Giza 86 and Giza 85 in El-Sharkia, El-Gharbiya, El-Monofiya and El-Dakahliya. This could be attributed to the growing locations had the same environmental conditions (nearly the same latitude). 3. Giza 88 exhibited the highest gin-stand capacity in Kafr El-Dawar growing location, while exhibited the lowest gin-stand capacity in Etay El-Barood growing location. Also, Giza 87 exhibited the highest gin-stand capacity in Kafr El-Dawar growing location, while exhibited the lowest gin-stand capacity in Damietta growing location. This could be attributed to the difference in cotton cultivar behavior and growing the cotton cultivar in different location led to increasing the environmental variations, consequently increasing the effect of plant location on cotton characteristics. 4. It is quite clear from the results that the fiber maturity and fiber fineness expressed as maicronaire value are the most fiber properties affecting gin stand capacity with lint grade, fiber length and fiber strength.