Potato leaf roll virus (PLRV) was isolated from Egyptian grown potatos. The virus was identified on the basis of host range, symptomatology, insect transmission, electron microscopy, RT-PCR, and PCR-ELISA. The complete nucleotide sequence of genomic PLRV-RNA was obtained from cloned cDNA and submitted in GenBank under Accession No. AY138970. This sequence is 5884 nucleotide long and encodes 5 ORF with an unique read-through protein suggested a conflict with the conceptual translation at amino acid 209. Comparison of PLRV sequence with that of other PLRV strains shows overall similarities of 97.02% and high genetic identity of 100% (Polish Strain), 98.5% (French Strain), 98.4% (Wageningen Strain), 98.3% (UK Strain), 98% (Canadian Strain), and 93. 5% (Australian Strain).