Application of 10 µM of Jasmonic acid (IA) as seed soaking significantly reduced % of root rot incidence in bean plants. Production of reactive oxygen species especially H2O2 was highly increased in both roots and shoots of R. solani infected-plants, as compared with non-infected control. JA significantly decreased the level of H2O2 in roots of 14 and 28 days old plants. Lipid peroxidation decreased in JA-treated plants, as compared with infected control. Activity of antioxidant enzymes (catalase, ascorbate peroxidase and superoxide dismutase) significantly increased in bean tissues in response to both pathogenic fungi (R.solani) and/or elicitor JA. JA-treated plants significantly increased total phenolic compounds and various phenolic acids except gallic acid in bean roots as compared with infected or noninfected control. Coumarin was induced only by JA. Additionally, activities of POX, PPO and PAL were greatly increased in both roots and shoots of bean plants infected by Rhizoctonia solani. JA treated plants showed the highest enzymes activity.