This study was conducted to investigate the effect of certain natural substances against Varroa mite. Four natural substances (Apiguard and Thyme, Camphor and Basil oils) were evaluated in the field as control agents against the parasitic mite, V. destructor. Four treatments of each tested material were applied to each colony during the treatment period. . Data showed that all four tested compounds were effective in controlling Varroa and Apiguard was the most effective substance as they had an efficiency of 81.3%, 74.6%, 71% and 67%, respectively.