Cross-inoculation studies of fifteen Pestalotiopsis isolates collected from different hosts and locations revealed that the fungus is a non specific pathogen. Isolates were able to attack apple, avocado, date-palm, guava, mango and pomegranate causing leaf spot and fruit rot. Disease severity and disease symptoms varied according to plant species. Guava plants was the most susceptible host, all being attacked by tested isolates resulting in brown leaf spot followed by pomegranate, while date palm was least affected. Avocado isolate (P6) was more aggressive on all tested plants, while date palm isolate (P12) was weakest. Six primers were used to determine the degrees of similarity among the tested isolates through RAPD - PCR. Isolates from the same host were not phylogenetically close - related. A close phylogenetic relationship among isolates possessing similar morphological characters was observed