Tissue culture inactivated ISA-70 oil adjuvanted EHV-1 vaccine was successfully prepared from the local isolate. The virus was completely inactivated by 0.008 M of binary ethyleneimine (BEI) within 24 hours at 37ºC. Mineral oil (ISA-70) achieves long term immunity & good seringeabilty. The vaccine proved to be safe, immunogenic in the inoculated mice & pregnant mares. It was potent in vaccinated horses with protective level of neutralizing & complements fixing and AGPT antibodies for 5 months. The duration of virus recovery in vaccinated mice after challenge was 4 days compared with 9 days in the control group. The stability of the prepared vaccine was studied whereas it could be stored at 4ºC, and withstand room temperature and 37ºC for 2 years.