The data used in this study were collected from two milking buffalo's farms .These farms are located at different geographical regions, one in the old land (village) and the other in reclaimed land (new land) in Alkobabat village, Atfeh city, Giza governorate. 50 dairy buffalo cows (3th and 4th parity), 25 animals in each, the first (farm 1) in the village and the second (farm 2) in the reclaimed land called Abou-Sroor land. The objective of the present investigation was to describing the lactation curve, calculate the persistency of lactation, total milk yield, daily milk yield and lactation period, and also to study physiological response for buffaloes under environmental conditions in the old land (village) and the reclaimed land. The results, found that the total milk yield and daily milk were higher in farm 1 than in farm 2. The daily milk yield (kg/day) was higher nearly 1 kg. Farm 2, have salinity in drinking water. The persistency value was higher in farm 2, although short lactation period were observed. Accordingly, buffalo breeding in the reclaimed land give good milk production traits.