This study was conducted to clarify effect of various treatments applied to control honey bees diseases and parasites on the Royal Jelly (RJ) sugar levels. RJ collected from untreated honey bee colonies with any kinds of treatments was contains lower values of the mono and disaccharides (Glucose, Fructose and Sucrose –Maltose) in comparison with other treatments as the Tylosin whose recorded higher levels with all tested sugars, while with Flagyl treatment it recognized by the highest value of the Glucose sugar, whereas with Thymol treatment it recorded the supreme level of the Sucrose sugar.