Experiments of this investigation were carried out at the experimental farm of the New Valley Agricultural Research Station, Agricultural Research Center, Egypt, during 2009/2010 and 2010/2011 seasons. The objective was to estimate the general (GCA) and specific combining abilities (SCA) of twelve genotypes for some quantitative characters in bread wheat using line x tester analysis. Twelve bread wheat genotypes were crossed to three local wheat cultivars (Sakha-93, Gemmeiza-9 and Sids-12) to produce thirty six test crosses. The studied characters were plant height, spikes per plant, kernels per spike, spike kernel weight, 100-kernel weight and grain yield per plant. Results indicated that, the genotypes (parents and crosses) exhibited significant variance for all studied characters, indicating the presence of genotypic differences. Mean squares due to parents vs. crosses were significant for all studied characters, except for spike kernel weight. The GCA/SCA ratio did not exceed unity for all studied characters, indicating that SCA variance was more important than GCA variance and thus non-additive genetic variance was the predominant component that controls the inheritance of all studied characters. Results indicated that Sids-12 could be recommended as a parental genotype for wheat breeding programs. The lines number two and nine had The highest GCA effects were shown by line 2 for spikes per plant, kernels per spike, line g for spike kernel weight and grain yield per plant. The lines and testers which showed higher combining ability effects for grain yield were also good combiners for at least one of the three yield components.