The experimental trial was consummated throughout two successive seasons (2010 and 2011) at Ornamental Plants Research and Landscape Dep., Hort. Res. Inst, Giza. It intended to find out the individual effect of mycorrhizal colonization and yeast or the combination between both as a biofertilizer. Besides the combined effect with half dose of NPK as preharvest treatments on ethylene production by flowers and keeping quality of cut flowers of Lilium asiaticum cv. Nello. The obtained results showed that all treatments significantly stimulated most of the studied characters compared with that gained from control plants. The inoculation of Lilium with either AM fungi and /or yeast supported the highest values of flower vase life and longevity of flowers and leaves. The addition of AM fungi solely or combined with yeast and/ or ½ NPK gave the maximum flower opening percentage, increased flower diameter, water uptake as well as the percentage of increase in fresh weight. The same combination revealed that leaves had higher total chlorophylls content and the flowers had higher total sugars%. In respect to ethylene production, it was noticed that Mycorrhizal colonization either solely or in combination with yeast and/or ½ NPK decreased flower ethylene production. Thus, mycorrhizal colonization may be a viable alternative to toxic ethylene inhibitors such as silver thiosulfate, since STS has proven to be environmental pollutant.