A survey of fungal diseases on alfalfa (cv. Sewa) in Ismailia governorate was carried out during 2011 and 2012. Percentages of infection were determined in five districs. Ismailia district, however, showed the highest percentage of infection by root rot / wilt disease and foliage diseases (0.0-60.0 %). Data indicated that the major fungal diseases affecting alfalfa in Ismailia governorate are damping-off and root-rot caused by Rhizoctonia solani and vascular wilt caused by Fusarium oxysporum and Vertcillium albo-atrum. The most important fungal foliar diseases were black stem (Phoma medicaginis) anthracnose (Colletotrichum trifolii), leaf spot (Stemphylium botryosum and Alternaria sp.) , Downy mildew (Peronospora sp.) and rust (Uromyces trifolii ). On the other hand, R. solani gave the highest percentage in isolation trials from alfalfa seeds followed by F. oxysporum, while Macrophominia phsolina was the least isolated . Also, R. solani was the most pathogenic fungus to alfalfa seeds in laboratory, followed by S. sclerotiorum, while M. phaseolina was the least.