irulence of three local isolates of entomopathogenic fungi ; Beauveria bassiana (AUMC 9896), Lecanicillium antillanum (=Verticillium antillanum) (AUMC 9905) and Paecilomyces lilacinus (AUMC 9897), as well as, Metarhizium anisopliae which isolated in Bio-insectcide Production Unit, Plant Protection Research Institute were evaluated against Spodoptera littoralis larvae under laboratory conditions. Five concentrations of spores suspension from each of the four fungal isolates (1×105, 1 ×106, 1×107, 1×108 and 1×109 conidia / ml) were used against newly hatched larvae of S. littoralis pest. The results showed that the percentage mortality of the larvae increased with increasing concentrations of tested pathogens. The median time of mortality decreased as the spores concentration increased. Also, the isolate B. bassiana was the most effective against S. littoralis larvae at different tested concentrations. B. bassiana at high concentration of 1.0x109 revealed 88.5 % mortality within mean time to larval mortality 5.1 ± 1.9 days. In biological Studies all tested fungi decreased the mean larval duration about 24 hours than untreated larvae and decreased of pupation. L. antillanum recorded the least Pupation percentage 45%. All tested fungi showed significantly shortening in the mean adult longevity for both males and females. All tested fungi significantly decreased the mean number of eggs laid and hatched /female.