the two-spotted spider mite Tetranychus urticae is considered to be one of the most economically important pests ‚ since it infests over 200 species of plants (Johnson 1991). Beauveria bassiana is highly control agent to this mite ‚and affects its mortality ‚biochemistry and physiolology. To test this effect ‚the B. bassiana was applied at 105 spores ⁄ ml using dipping leaf -disc treatment method. Mortality, total proteins, carbohydrates and lipids of mite were measured; Mites adult females were susceptible to B. bassiana, with high mortality rate recorded. The effect of B. bassiana on proteins level in the mycosed extract homogenate appeared decreasing than the nonmycosed during the period of experiment. The means of total protiens are 225‚220 and 183 μg⁄ml after 24‚48 and 72hrs respectively‚compared with327 μg⁄1000 individuals in untreated control. Effect of the B. bassiana on the level of the carbohydrates decreased in the treated extract homogenate than the untreated one during the period of experiment. The mean of total carbohydrates at 24‚48 and 72 hrs after treatment were 142‚138 and 115 μg⁄ml respectively‚ compared with149 μg⁄1000 ml‚ In untreated control. The effect of B. bassiana on lipids level in treated extract homogenate appeared decreasing than in untreated one during the period of experiment. The obtained result refer to the mean total lipids were 92‚77‚ and 70 μg⁄ ml after 24‚48 and 72 hrs ‚ respectively compared with 88 μg⁄1000 individuals untreated adult females of T. urticae. This study improves that entomopathogenic fungus B. bassiana causes many biochemical and physiological changes in the mite Tetranychus urticae. B. bassiana is also considered as a safe biological control agent to the environment.