The tomato leaf miner; Tuta absoluta (Meyrick) is one of the most important pests of tomato all over the world. Choosing of tomato cultivar plays an important role in controlling this insect pest. The susceptibility of some tomato cultivars to infestation with this pest in relation to leaflet trichomes was studied. Five tomato cultivars; Alissa F1, Super strain B, G.S 12 F1, E603 F1(Logain) and Indos were planted in both early summer and summer plantations. Results indicated that the most susceptible cultivars to the infestation were Alissa F1 and Super strain B while Logain cultivars was the least susceptible one. Two trichome types were detected in all tomato cultivars leaflet; one of them is glandular and the other is non glandular. Glandular trichomes length and density showed high significant negative correlation with T. absoluta infestation and length of normal trichomes showed significant negative correlation. So it is suggested that density of glandular trichomes might plays an important role in tomato cultivars susceptibilities.