Asurvey on mites associated with stored onion bulbs at El- Menofia governorate during 2014 and 2015 years, proved the occurrence of 21 different species. Of these, eight species from two families Acaridae and Glycyphagidae belong to Astigmata, six species belong to five families (Bdellidae, Rhagididae, Cheyletidae, Tydeidae and Neophyllobidae) under Prostigmata, 6 species in 3 families (Ascidae, Laelapidae and Ameroseiidae) belong to Mesostigmata and one mite species in one family (Oppiidae) under Suborder Cryptostigmata. Members of the families Acaridae, Glycyphagidae and Cheyletidae were the most common mites, found in many samples with high abundance. The storage conditions affected on the abundance of different collected mites, as Rhizoglyphus echinopus, Tyrophagus putrescentiae, Caloglyphus berlesei (Family Acaridae), Glycyphagus ornatus (Family Glycyphagidae); and Cheyletus eruditus, Family (Cheyletidae); were recorded from the different three storage conditions. On the other hand, Lardoglyphus konoi, Lardoglyphus zacheri, Tyrophagus tropicus, Spinibdella bifurcata, Coccorhagidia clavifron, Lasioseius eagypticus, Lasiosius lindiquisti, Androlaelaps wahabi, Kleemenia plumosus and K. zaheri were collected from the bulbs which exposed to straw covered bulbs condition. On the other hand, Pronematus rykei (Tydeidae) and Neophyllobius aegyptium (Neophyllobidae) were identified species when onion bulbs stored with opened and shady room condition; while Cheyletus badryi (Cheyletidae) was isolated from onion bulbs stored at straw covered bulbs and Indoor storage conditions.