Quality of the Royal jelly (RJ) is a reflection of the hypopharyngeal glandular (HG) physiological state. Faraway it depends on the feeding protein and lipids qualitative during and after bee workers maturation. Effect of sustain honey bee colonies by some nutrition process on the (HG) secretion were investigated using HPLC analysis. Honey bee colonies fed one of the 4 treatments (Brewer's Yeast, Soybean flour, Date palm pollen (Phoenix dactylifera) and Pollen grains from other plants); further control treatment. The results indicated presence a clear amelioration in the RJ quality phenols. 1-The tested Benzoic phenol with Date palm pollen treatment recorded the lowest concentration (2.65mg/100g) in comparable to control one (6.88mg/100g) and lower than others, that is important for public health. 2- The Caffeine phenol restricted the highest record with the pollen grains treatment (3.90mg/100g) in comparable with the control (0.95mg/100g) that crucial for memory. 3- The highest concentration of the Ellagic phenol was recorded with pollen grains treatment (9.55mg/100g). 4- Ferulic phenol recorded higher concentration only with Brewer's Yeast treatment (1.31mg/100g). 5- Higher density of the (RJ) secretion inside the secret vesicles lobules with Date palm pollen followed with Brewer's Yeast treatments was detected. This paper summary to produce high quality royal jelly one must consider food technology for honeybee colonies in feed diets.