In rodent control programs House mouse, Mus musculus appear high tolerance to anticoagulant rodenticide. So, susceptibility of M. musculus trapped from houses and vegetable field habitats at warfarin rodenticide bait alone or mix with astonin-H (Fludrocortisone) to improve the warfarin efficiency was tested in Beni-suef Governorate. Under laboratory conditions astonin addition to warfarin bait improve the mortality percent, with concentrate astonin, the mortality were 80%, 90%, 100% and 100% to warfarin bait 0.025% + 0.1, 0.25, 0.5 and 1.0% astonin respectively compared with warfarin bait 0.025% alone (50%). Tested mice of M. musculus trapped from houses were susceptible to warfarin alone than trapped from fields the lethal feeding period, (LFP50 and LFP90) to each treatment war 4.8 & 10.8) and 5.6 & 15.0 day respectively. The LFP50 and LFP90 to warfarin bait + astonin decrease to (3.2, 6.3) and (3.6, 8.5) day to mice trapped houses and field compared with the same treatment with warfarin bait alone, all's, the time to death and bait consumptions reduced to near 40% with astonin addition. Under field conditions the population reductions were 86 and 91% in vegetable field and houses with warfarin bait 0.025% + astonin 0.25%. In the same habitats warfarin bait 0.025% alone caused 64% and 68% reduction in population mice to each habitat respectively. So that the present work was directed to study the importance anticoagulant rodenticide treatment by addition astonin to improve the anticoagulant rodenticide efficiency, abate the quantity of using bait, control time and effort in rodent programs. I