The present investigation deals with comparison among some Egyptian cotton genotypes (Gossypium barbadense L.) with respect to yield and its components in four locations (Fayuom, Minia, Assuit and Sohag) in Upper Egypt during 2014 and 2015 seasons using different statistical analyses. Genotypes were G.80, G.90, (G.83x(G.75x5844))xG.80 and [(G.83xG.80)xG89] x Australum. A randomized complete block design was used in each location. The first step of comparative was analysis of individual locations. (G.83xG.80)xG.89]xAustralum significantly surpassed other genotypes in Fayuom (L1) in the two seasons except G90 in the 2014 season with respect to seed cotton yield. The results of lint cotton yield exhibited the same genotype was the best genotypes in Fayuom (L1) in the two seasons and Minia (L2) in 2014 season. [G.83 x (G.75 x 5844)] x G.80 significantly exceeded other genotypes with respect to boll weight in Sohag (L4) in the first season. On the other hand, G80 significantly surpassed all genotypes with respect to the same trait in Fayuom (L1) and Sohag (L4) in the second season. [(G.83xG.80)xG.89]xAustralum significantly surpassed other genotypes with respect to lint percentage in three locations [ Fayuom (L1), Minia (L2) and Sohag (L4) ] except [G.83 x (G.75 x 5844)] x G.80 in Sohag (L4) in the two seasons. In the two seasons, G.80 significantly surpassed other genotypes with respect to seed index in Sohag (L4) except G.90 in the first season. In the two seasons, non - significant variation due to genotypes was observed for lint index in all locations except Assuit (L3) in the second season. Combined analysis was made to estimate the locations and the genotypes x locations effects on cotton. The genotypes x locations was non-significant with respect to yield and its components in the first season except seed cotton yield. On the contrary, the genotypes x locations was significant with respect to lint cotton yield, boll weight and lint percentage in the second season. The third analysis was made to estimate the environments and genotypes x environments effect on cotton. The interaction between genotypes x environments was significant with respect to yield and its components except seed and lint index. The present study is very important for the regional program to evaluate the genotypes suitable for cultivation in Upper Egypt.