This study investigated Pseudomonas fluorescens as a potential biological control agent of Tetranychus cucurbitacearum by using two different methods; spraying and dipping. The earliest death occurred within the first day after the treatments. The highest mortality (100%) and the lowest 68.75% for the spraying bacterial treatment and 58.75% and 16.25% for the dipping. The longevity was shorter than control in both methods. Female longevity averaged 10.32 and 17.04 days for the spraying and the dipping while, 22.5 and 24.19 days in control. The bacterial treatment reduced the total deposited eggs in females to half approximately in the spraying method while, in the dipping method was nearly with the control. The chitinase enzyme present in the cell was confirmed by the positive result of chitin clearing zone.