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152587

EFFICACY OF SECONDARY METABOLITES AND EXTRACELLULAR LYTIC ENZYMES OF PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR) IN CONTROLLING FUSARIUM WILT OF CHICKPEA

Article

Last updated: 24 Dec 2024

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Abstract

Plant growth promoting rhizobacteria (PGPR) and its metabolic products may play a pivotal role in controlling wilt disease in chickpea plants caused by Fusarium oxysporum f. sp. ciceris and promote plant growth under greenhouse and field conditions. The obtained data indicated that both PGPR strains viz. Bacillus subtilis and B. megaterium were able to produce indole acetic acid (IAA), siderophore, hydrogen cyanide (HCN), extracellular compound and volatile antibiotics in vitro. In addition, both PGPR strains produced mycolytic enzymes viz. chitinase, β-1, 3-glucanase and protease in growth media. Bacillus megaterium produced greater amounts of secondary metabolites than B. subtilis. Under laboratory condition, cell cultures, culture filtrates, metabolic precipitates with acetone, ethanol and ammonium sulfate inhibited mycelial growth of the target pathogen and the percentage of inhibition varied from 26.07 % to 48.82 %. Bacillus megaterium inhibited mycelial growth more than B. subtilis. Also, the metabolic precipitate significantly suppressed mycelial growth of pathogen more than cell cultures or culture filtrates and the ethanol precipitate was the most inhibitive. Under greenhouse and field conditions, both PGPR strains used as seed soaking in cell cultures, culture filtrates and/or metabolic precipitates significantly reduced area under wilt progress curve (AUWPC) compared with untreated seeds (control). B. megaterium was more efficient for controlling wilt disease in chickpea than B. subtilis and the metabolic precipitates reduced AUWPC more than cell cultures or its filtrates. Ethanol precipitate recorded the highest reduction of AUWPC either under greenhouse or field conditions. Using cell cultures, cultures filtrates and metabolic precipitates of both PGPR strains significantly increased growth parameters (plant height and number of branches per plant), yield components (numbers of pod and seed /plant, weight of 100 seed, total yield / feddan and protein content in seeds) compared with untreated seeds (control) during growing seasons 2013-14 and 2014-15 under field conditions. Ethanol precipitate of B. megaterium or B. subtilis recorded the highest growth parameters and yield components in both growing seasons. Generally, metabolic precipitation recorded the best results for controlling wilt disease under greenhouse and field conditions and improved plant growth and increased yield components more than cell cultures or culture filtrates of both tested PGPR strains. 

DOI

10.21608/ejar.2016.152587

Keywords

chickpea, Fusarium oxysporum f. sp. ciceris, HCN, Growth parameters and yield components, IAA, Mycolytic enzymes, PGPR, Secondary metabolic, Siderophores

Authors

First Name

MONTASER F.

Last Name

ABDEL-MONAIM

MiddleName

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Affiliation

Plant Pathology Research Institute, Agricultural Research Center, 12619, Giza Egypt

Email

fowzy_2008@yahoo.com

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Orcid

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Volume

94

Article Issue

3

Related Issue

22492

Issue Date

2016-09-01

Receive Date

2016-03-24

Publish Date

2016-09-01

Page Start

573

Page End

589

Print ISSN

1110-6336

Online ISSN

2812-4936

Link

https://ejar.journals.ekb.eg/article_152587.html

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https://ejar.journals.ekb.eg/service?article_code=152587

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1

Type

Original Article

Type Code

1,041

Publication Type

Journal

Publication Title

Egyptian Journal of Agricultural Research

Publication Link

https://ejar.journals.ekb.eg/

MainTitle

EFFICACY OF SECONDARY METABOLITES AND EXTRACELLULAR LYTIC ENZYMES OF PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR) IN CONTROLLING FUSARIUM WILT OF CHICKPEA

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Article

Created At

22 Jan 2023