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148458

BIOGENIC SILICA NANOPARTICLES, SYNTHESIS, CHARACTERIZATION AND ANTIFUNGAL ACTIVITY AGAINST TWO RICE PATHOGENIC FUNGI

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Last updated: 24 Dec 2024

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Abstract

silica nanoparticles (Si NPs) were extracted by different methods from mrice husk (RH)and ric estraw (RS), and characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). For the XRD results, the crystalline size was calculated using Scherer equation. The particle size of rice husk nanoparticles (RHNPs) and rice straw nanoparticles (RSNPs) were 73.6 nm and 133.7 nm, respectively. Silica present in RHNPs and RSNPs was about 22.78 and 9.56%, respectively. Si NPs were effective in controlling rice blast (Pyricularia grisea) and brown spot (Bipolaris oryzae) fungal diseases under greenhouse conditions during 2015 season. Efficiency of soil application with RHNPs and white rice husk ash WRHA 92.56 & 90.90% at the rate of 1.5 g/1kg, were the most effective treatments to reduce blast disease severity compared to other treatments as well as control. On the other hand, rice plantswere treated with liquid potassium silicate (K2SiO2), WRHA and RHNPs gave 96.92, 93.07 and 91.53 % efficiency, respectively as foliar application for the control of brown spot disease compared with other treatments. SEM/energy-dispersive spectrometer (EDX) observations and X-ray spectra of adaxial surfaces of the fourth rice leaves Sakha 101 rice cultivar) in soil applied with 1.5g/1kg gave different types of silicified cells. The corresponding EDX spectra compared with the SEM images demonstrated differences in silicon content between soil treated by RHNPs 13.75% and nontreated plants 10.6%. Silicon accumulation in Sakha 101 rice leaves treated with RHNPs as soil application at 1.5g/1kg was increasing Si layers in epidermal cell walls, cuticle and the thickness of the silicon layer. Also, outer regions of epidermal cell walls and intercellular spaces within sub-epidermal tissues. All the silicon layers may be playinga role in increasing the resistanceof rice plants and controlling the rice diseases.

DOI

10.21608/ejar.2017.148458

Keywords

Rice husk - straw- Silica, NPs- Pyricularia grisea- Bipolaris oryzae

Authors

First Name

ZEINAB A.,

Last Name

KALBOUSH

MiddleName

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Affiliation

Rice Pathol.Dept., Plant Pathol. Res. Inst., ARC, Egypt.

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First Name

WAEL EL.

Last Name

GABR

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Affiliation

Rice Pathol.Dept., Plant Pathol. Res. Inst., ARC, Egypt.

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First Name

AMRO A.

Last Name

HASSAN

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-

Affiliation

Rice Pathol.Dept., Plant Pathol. Res. Inst., ARC, Egypt.

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Volume

95

Article Issue

2

Related Issue

21693

Issue Date

2017-07-01

Receive Date

2017-01-10

Publish Date

2017-07-01

Page Start

543

Page End

558

Print ISSN

1110-6336

Online ISSN

2812-4936

Link

https://ejar.journals.ekb.eg/article_148458.html

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https://ejar.journals.ekb.eg/service?article_code=148458

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2

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Original Article

Type Code

1,041

Publication Type

Journal

Publication Title

Egyptian Journal of Agricultural Research

Publication Link

https://ejar.journals.ekb.eg/

MainTitle

BIOGENIC SILICA NANOPARTICLES, SYNTHESIS, CHARACTERIZATION AND ANTIFUNGAL ACTIVITY AGAINST TWO RICE PATHOGENIC FUNGI

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Article

Created At

22 Jan 2023