infectious bronchitis virus is an acute and economically important viral disease of chickens. IB is a member of the Coronaviridae family it is an acute disease of respiratory and urogenital tract of chickens. Some IBV strains can cause acute nephritis and urolithiasis associated with the high mortality rate in chickens; considering (IBV) which can affect avian and represent a risk for public health, classified as risk group 2. This study, take place in isolators to detect of experimental waste condemnation after challenge variant IB strain in 3 weeks specific pathogen-free vaccinated birds and non-vaccinated control. Samples (kidneys and lungs) were collected for the detection of pathological changes. No Histopathological lesions were observed, nor virus was detected in the following challenge. This work was designed to apply biosecurity and biosafety parameter which including biological waste decontamination and disinfection in order to judge about any residual living virus after challenge test