the present work was carried out to evaluate the toxic and latent effects of Chromafenozide on the eggs, larval and adult stages of Chrysoperla carnea (Stephens) under laboratory conditions. Results indicate that the LC20 and LC50 values of Chromafenozide for 1-2 days old adults were 58.40 and 135.75 ppm, respectively. Highly reduction of adults (males and females) longevity, the mean number of eggs/female/day and percentage of eggs hatchability were observed. Application on eggs resulted from treated adults represented LC50 value 62.24 ppm, while eggs from untreated adults gave 93.34ppm. The results show the direct effect of Chromafenozide on incubation periods and percentage of hatchability, as well as, the latent effect on larval duration, and cocoons duration were also highly significant affected and a reduction in adult emergence from both egg treatments with an increase in malformation by 43.7 and 27.8% compared to 0% in control and highly affected by treatment, when reared on prey; Pectinophora gossypiella (Saund.) eggs. Also, the biochemical study shows a clear reduction in the total protein of larvae reared on eggs treated with Chromafenozide reduced the total protein content to -22.53% compared with the control larvae. The phenoloxidase which necessary in melanin production during cuticle content, it decreased to -46.04% in the treatment. Which due to changes in N-acetylglucos-amine reached to -26.91%, and the changes in chitinase decreased to -4.68% in treatment compared with control.